Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1732197..1732738 Replicon chromosome
Accession NZ_CP027454
Organism Escherichia coli strain 2014C-4423

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag C6W74_RS08885 Protein ID WP_000615976.1
Coordinates 1732197..1732475 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID A0A4T9JVH9
Locus tag C6W74_RS08890 Protein ID WP_000729706.1
Coordinates 1732478..1732738 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W74_RS08870 1729779..1730357 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
C6W74_RS08875 1730563..1731330 + 768 WP_000333380.1 class II glutamine amidotransferase -
C6W74_RS08880 1731301..1732041 - 741 WP_001225679.1 murein L,D-transpeptidase -
C6W74_RS08885 1732197..1732475 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C6W74_RS08890 1732478..1732738 - 261 WP_000729706.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C6W74_RS08895 1732924..1733697 + 774 WP_000543899.1 C40 family peptidase -
C6W74_RS08900 1733754..1734110 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C6W74_RS08905 1734103..1734381 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C6W74_RS08910 1734486..1736198 - 1713 Protein_1613 flagellar biosynthesis protein FlhA -
C6W74_RS08915 1736170..1736955 + 786 WP_000207558.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T98960 WP_000615976.1 NZ_CP027454:c1732475-1732197 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T98960 NZ_CP027454:c1732475-1732197 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9390.79 Da        Isoelectric Point: 5.8129

>AT98960 WP_000729706.1 NZ_CP027454:c1732738-1732478 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADNL
FDKLGI

Download         Length: 261 bp

>AT98960 NZ_CP027454:c1732738-1732478 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATAATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
AlphaFold DB A0A4T9JVH9

References