Detailed information of TA system TA098957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 779657..780259
• Replicon: chromosome
• Accession: NZ_CP027454
• Organism: Escherichia coli strain 2014C-4423

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6W74_RS04030
• Protein ID: WP_000897305.1
• Coordinates: 779657..779968 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6W74_RS04035
• Protein ID: WP_000356397.1
• Coordinates: 779969..780259 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W74_RS04000	774701..775138	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6W74_RS04005	775183..776124	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6W74_RS04010	776488..777426	+	939	Protein_731	hypothetical protein	-
C6W74_RS04015	777439..778353	+	915	WP_072097029.1	transposase	-
C6W74_RS04025	778521..779428	-	908	Protein_733	alpha/beta hydrolase	-
C6W74_RS04030	779657..779968	+	312	WP_000897305.1	hypothetical protein	Toxin
C6W74_RS04035	779969..780259	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6W74_RS04045	780845..781063	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6W74_RS04050	781282..781524	+	243	WP_001086388.1	hypothetical protein	-
C6W74_RS04055	781854..782783	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6W74_RS04060	782780..783415	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6W74_RS04065	783412..784314	-	903	WP_016233699.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98957 WP_000897305.1 NZ_CP027454:779657-779968 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98957 NZ_CP027454:779657-779968 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98957 WP_000356397.1 NZ_CP027454:779969-780259 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98957 NZ_CP027454:779969-780259 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7