Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2691632..2692431 Replicon chromosome
Accession NZ_CP027452
Organism Escherichia coli strain 2014C-3338

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag C6W71_RS13980 Protein ID WP_000347251.1
Coordinates 2691632..2692096 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag C6W71_RS13985 Protein ID WP_001296435.1
Coordinates 2692096..2692431 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W71_RS13945 2686633..2687067 - 435 WP_000948823.1 PTS sugar transporter subunit IIA -
C6W71_RS13950 2687085..2687963 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
C6W71_RS13955 2687953..2688732 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
C6W71_RS13960 2688743..2689216 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
C6W71_RS13965 2689239..2690519 - 1281 WP_000681938.1 tagatose-bisphosphate aldolase subunit KbaZ -
C6W71_RS13975 2690768..2691577 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
C6W71_RS13980 2691632..2692096 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
C6W71_RS13985 2692096..2692431 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
C6W71_RS13990 2692580..2694151 - 1572 WP_001273939.1 galactarate dehydratase -
C6W71_RS13995 2694526..2695860 + 1335 WP_000979025.1 galactarate/glucarate/glycerate transporter GarP -
C6W71_RS14000 2695876..2696646 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T98941 WP_000347251.1 NZ_CP027452:c2692096-2691632 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T98941 NZ_CP027452:c2692096-2691632 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT98941 WP_001296435.1 NZ_CP027452:c2692431-2692096 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT98941 NZ_CP027452:c2692431-2692096 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References