Detailed information of TA system TA098934

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1852442..1853044
• Replicon: chromosome
• Accession: NZ_CP027452
• Organism: Escherichia coli strain 2014C-3338

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6W71_RS09630
• Protein ID: WP_000897305.1
• Coordinates: 1852733..1853044 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6W71_RS09625
• Protein ID: WP_000356397.1
• Coordinates: 1852442..1852732 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W71_RS09605	1848944..1849846	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
C6W71_RS09610	1849843..1850478	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6W71_RS09615	1850475..1851404	+	930	WP_052920312.1	formate dehydrogenase accessory protein FdhE	-
C6W71_RS09620	1851619..1851837	-	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
C6W71_RS09625	1852442..1852732	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6W71_RS09630	1852733..1853044	-	312	WP_000897305.1	hypothetical protein	Toxin
C6W71_RS09635	1853273..1854181	+	909	WP_001318161.1	alpha/beta hydrolase	-
C6W71_RS09640	1854245..1855186	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6W71_RS09645	1855231..1855668	-	438	WP_021560109.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6W71_RS09650	1855665..1856537	-	873	WP_000920762.1	virulence factor BrkB family protein	-
C6W71_RS09655	1856531..1857130	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98934 WP_000897305.1 NZ_CP027452:c1853044-1852733 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98934 NZ_CP027452:c1853044-1852733 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98934 WP_000356397.1 NZ_CP027452:c1852732-1852442 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98934 NZ_CP027452:c1852732-1852442 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7