Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4063402..4063904 Replicon chromosome
Accession NZ_CP027449
Organism Escherichia coli strain 2014C-3097

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6W73_RS20775 Protein ID WP_000767829.1
Coordinates 4063650..4063904 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6W73_RS20770 Protein ID WP_001259255.1
Coordinates 4063402..4063653 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W73_RS20740 4058580..4059647 - 1068 WP_000080130.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C6W73_RS20745 4059647..4060717 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
C6W73_RS20750 4060714..4062018 - 1305 WP_001356062.1 histidinol dehydrogenase -
C6W73_RS20755 4062024..4062923 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6W73_RS20765 4063069..4063119 - 51 WP_001364200.1 his operon leader peptide -
C6W73_RS20770 4063402..4063653 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6W73_RS20775 4063650..4063904 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6W73_RS20780 4063987..4064811 + 825 WP_000754758.1 SDR family oxidoreductase -
C6W73_RS20785 4064857..4065786 + 930 WP_106879140.1 LysR family transcriptional regulator -
C6W73_RS20790 4066001..4066063 + 63 WP_010723108.1 membrane protein YoeI -
C6W73_RS20795 4066053..4067411 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6W73_RS20800 4067590..4068648 + 1059 WP_000492318.1 transport protein YeeE -
C6W73_RS20805 4068662..4068889 + 228 WP_000985280.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98911 WP_000767829.1 NZ_CP027449:4063650-4063904 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98911 NZ_CP027449:4063650-4063904 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98911 WP_001259255.1 NZ_CP027449:4063402-4063653 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98911 NZ_CP027449:4063402-4063653 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References