Detailed information of TA system TA098904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1954586..1955188
• Replicon: chromosome
• Accession: NZ_CP027449
• Organism: Escherichia coli strain 2014C-3097

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6W73_RS10055
• Protein ID: WP_000897305.1
• Coordinates: 1954877..1955188 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6W73_RS10050
• Protein ID: WP_000356397.1
• Coordinates: 1954586..1954876 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W73_RS10020	1950512..1951414	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
C6W73_RS10025	1951411..1952046	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6W73_RS10030	1952043..1952972	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6W73_RS10035	1953302..1953544	-	243	WP_001086388.1	hypothetical protein	-
C6W73_RS10040	1953763..1953981	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6W73_RS10050	1954586..1954876	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6W73_RS10055	1954877..1955188	-	312	WP_000897305.1	hypothetical protein	Toxin
C6W73_RS10060	1955417..1956325	+	909	WP_001162704.1	alpha/beta hydrolase	-
C6W73_RS10065	1956389..1957330	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6W73_RS10070	1957375..1957812	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6W73_RS10075	1957809..1958681	-	873	WP_000920762.1	virulence factor BrkB family protein	-
C6W73_RS10080	1958675..1959274	-	600	WP_001315111.1	glucose-1-phosphatase	-
C6W73_RS10085	1959373..1960158	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98904 WP_000897305.1 NZ_CP027449:c1955188-1954877 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98904 NZ_CP027449:c1955188-1954877 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98904 WP_000356397.1 NZ_CP027449:c1954876-1954586 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98904 NZ_CP027449:c1954876-1954586 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7