Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) hok-sok/-
Location 136414..136840 Replicon plasmid unnamed
Accession NZ_CP027448
Organism Escherichia coli strain 2014C-3075

Toxin (Protein)


Gene name hok Uniprot ID P11895
Locus tag C6W72_RS27225 Protein ID WP_001312861.1
Coordinates 136414..136572 (-) Length 53 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 136616..136840 (-)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W72_RS27175 131464..131691 - 228 WP_001254386.1 conjugal transfer relaxosome protein TraY -
C6W72_RS27180 131785..132471 - 687 WP_000332492.1 PAS domain-containing protein -
C6W72_RS27185 132661..133044 - 384 WP_000124980.1 relaxosome protein TraM -
C6W72_RS27195 133378..133968 + 591 WP_187143838.1 transglycosylase SLT domain-containing protein -
C6W72_RS27200 134265..135086 - 822 Protein_133 DUF932 domain-containing protein -
C6W72_RS27205 135205..135492 - 288 WP_106901846.1 hypothetical protein -
C6W72_RS27815 135517..135723 - 207 WP_069907064.1 single-stranded DNA-binding protein -
C6W72_RS27225 136414..136572 - 159 WP_001312861.1 type I toxin-antitoxin system Hok family toxin Toxin
- 136616..136840 - 225 NuclAT_0 - Antitoxin
- 136616..136840 - 225 NuclAT_0 - Antitoxin
- 136616..136840 - 225 NuclAT_0 - Antitoxin
- 136616..136840 - 225 NuclAT_0 - Antitoxin
C6W72_RS27685 136652..136840 + 189 WP_001299721.1 hypothetical protein -
C6W72_RS27230 136852..137571 - 720 WP_106901848.1 plasmid SOS inhibition protein A -
C6W72_RS27235 137568..138002 - 435 WP_032217500.1 conjugation system SOS inhibitor PsiB -
C6W72_RS27240 138057..140015 - 1959 WP_106901849.1 ParB/RepB/Spo0J family partition protein -
C6W72_RS27245 140080..140313 - 234 WP_106901850.1 DUF905 family protein -
C6W72_RS27250 140375..140914 - 540 WP_009426937.1 single-stranded DNA-binding protein -
C6W72_RS27820 140940..141146 - 207 WP_106901851.1 single-stranded DNA-binding protein -
C6W72_RS27265 141387..141626 - 240 WP_072163661.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyB / hlyA / hlyC / faeC / faeD / faeE / faeF / faeH / faeI 1..170848 170848


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(8-48)


Sequences


Toxin        


Download         Length: 53 a.a.        Molecular weight: 6082.32 Da        Isoelectric Point: 9.2584

>T98893 WP_001312861.1 NZ_CP027448:c136572-136414 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Download         Length: 159 bp

>T98893 NZ_CP027448:c136572-136414 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 225 bp

>AT98893 NZ_CP027448:c136840-136616 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB P11895


Antitoxin

Download structure file

References