Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4582935..4583476 Replicon chromosome
Accession NZ_CP027447
Organism Escherichia coli strain 2014C-3075

Toxin (Protein)


Gene name yafQ Uniprot ID D8A3N8
Locus tag C6W72_RS23445 Protein ID WP_000615978.1
Coordinates 4582935..4583213 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag C6W72_RS23450 Protein ID WP_000729709.1
Coordinates 4583216..4583476 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W72_RS23430 4580607..4581185 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
C6W72_RS23435 4581301..4582068 + 768 WP_000333380.1 class II glutamine amidotransferase -
C6W72_RS23440 4582039..4582779 - 741 WP_001225679.1 murein L,D-transpeptidase -
C6W72_RS23445 4582935..4583213 - 279 WP_000615978.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C6W72_RS23450 4583216..4583476 - 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C6W72_RS23455 4583662..4584435 + 774 WP_122999991.1 C40 family peptidase -
C6W72_RS23460 4584533..4586626 - 2094 WP_063081503.1 flagellar biosynthesis protein FlhA -
C6W72_RS23465 4586610..4587749 - 1140 WP_097291330.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.66 Da        Isoelectric Point: 10.0702

>T98888 WP_000615978.1 NZ_CP027447:c4583213-4582935 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLLLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T98888 NZ_CP027447:c4583213-4582935 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACTGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT98888 WP_000729709.1 NZ_CP027447:c4583476-4583216 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT98888 NZ_CP027447:c4583476-4583216 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACTATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G928

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References