Detailed information of TA system TA098851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4138044..4138771
• Replicon: chromosome
• Accession: NZ_CP027445
• Organism: Escherichia coli strain 2013C-3492

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: C6W68_RS22155
• Protein ID: WP_000547563.1
• Coordinates: 4138044..4138355 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6W68_RS22160
• Protein ID: WP_000126296.1
• Coordinates: 4138352..4138771 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W68_RS22125	4133186..4134895	+	1710	WP_047087242.1	formate hydrogenlyase subunit HycE	-
C6W68_RS22130	4134905..4135447	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
C6W68_RS22135	4135447..4136214	+	768	WP_032207579.1	formate hydrogenlyase subunit HycG	-
C6W68_RS22140	4136211..4136621	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
C6W68_RS22145	4136614..4137084	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
C6W68_RS22150	4137127..4137882	+	756	WP_032207575.1	hypothetical protein	-
C6W68_RS22155	4138044..4138355	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
C6W68_RS22160	4138352..4138771	+	420	WP_000126296.1	helix-turn-helix domain-containing protein	Antitoxin
C6W68_RS22165	4138886..4140310	-	1425	WP_032207736.1	6-phospho-beta-glucosidase AscB	-
C6W68_RS22170	4140319..4141776	-	1458	WP_047087240.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C6W68_RS22175	4142036..4143046	+	1011	WP_001374632.1	DNA-binding transcriptional regulator AscG	-
C6W68_RS22180	4143195..4143722	+	528	WP_032207572.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T98851 WP_000547563.1 NZ_CP027445:4138044-4138355 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T98851 NZ_CP027445:4138044-4138355 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAGATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.38 Da
• Isoelectric Point: 4.3653

>AT98851 WP_000126296.1 NZ_CP027445:4138352-4138771 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT98851 NZ_CP027445:4138352-4138771 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACCAAGAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22