Detailed information of TA system TA098847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3632309..3633108
• Replicon: chromosome
• Accession: NZ_CP027445
• Organism: Escherichia coli strain 2013C-3492

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: C6W68_RS19520
• Protein ID: WP_032208138.1
• Coordinates: 3632309..3632773 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: C6W68_RS19525
• Protein ID: WP_032208136.1
• Coordinates: 3632773..3633108 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W68_RS19485	3627310..3627744	-	435	WP_032208141.1	PTS sugar transporter subunit IIA	-
C6W68_RS19490	3627762..3628640	-	879	WP_001341890.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
C6W68_RS19495	3628630..3629409	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
C6W68_RS19500	3629420..3629893	-	474	WP_032208140.1	PTS N-acetylgalactosamine transporter subunit IIB	-
C6W68_RS19505	3629916..3631196	-	1281	WP_047087618.1	tagatose-bisphosphate aldolase subunit KbaZ	-
C6W68_RS19515	3631445..3632254	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
C6W68_RS19520	3632309..3632773	-	465	WP_032208138.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
C6W68_RS19525	3632773..3633108	-	336	WP_032208136.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
C6W68_RS19530	3633257..3634828	-	1572	WP_032208135.1	galactarate dehydratase	-
C6W68_RS19535	3635203..3636537	+	1335	WP_047087619.1	galactarate/glucarate/glycerate transporter GarP	-
C6W68_RS19540	3636553..3637323	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17837.19 Da
• Isoelectric Point: 9.4947

>T98847 WP_032208138.1 NZ_CP027445:c3632773-3632309 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGASRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T98847 NZ_CP027445:c3632773-3632309 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTAGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12405.01 Da
• Isoelectric Point: 4.8616

>AT98847 WP_032208136.1 NZ_CP027445:c3633108-3632773 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKLGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT98847 NZ_CP027445:c3633108-3632773 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCTAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA