Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1913788..1914329 Replicon chromosome
Accession NZ_CP027445
Organism Escherichia coli strain 2013C-3492

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag C6W68_RS10600 Protein ID WP_000615979.1
Coordinates 1914051..1914329 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C6W68_RS10595 Protein ID WP_000729704.1
Coordinates 1913788..1914048 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W68_RS10570 1909571..1910356 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
C6W68_RS10575 1910328..1912040 + 1713 Protein_1937 flagellar biosynthesis protein FlhA -
C6W68_RS10580 1912145..1912423 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C6W68_RS10585 1912416..1912772 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C6W68_RS10590 1912829..1913602 - 774 WP_000543899.1 C40 family peptidase -
C6W68_RS10595 1913788..1914048 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C6W68_RS10600 1914051..1914329 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C6W68_RS10605 1914485..1915225 + 741 WP_001225679.1 murein L,D-transpeptidase -
C6W68_RS10610 1915196..1915962 - 767 Protein_1944 class II glutamine amidotransferase -
C6W68_RS10615 1916168..1916746 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T98843 WP_000615979.1 NZ_CP027445:1914051-1914329 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T98843 NZ_CP027445:1914051-1914329 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT98843 WP_000729704.1 NZ_CP027445:1913788-1914048 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT98843 NZ_CP027445:1913788-1914048 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References