Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1324348..1324850 Replicon chromosome
Accession NZ_CP027442
Organism Escherichia coli strain 2013C-3252

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag MJ69_RS07960 Protein ID WP_000767829.1
Coordinates 1324348..1324602 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag MJ69_RS07965 Protein ID WP_001259255.1
Coordinates 1324599..1324850 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MJ69_RS07930 1319363..1319590 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
MJ69_RS07935 1319604..1320662 - 1059 WP_000492324.1 transport protein YeeE -
MJ69_RS07940 1320841..1322199 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
MJ69_RS07945 1322189..1322251 - 63 WP_010723108.1 membrane protein YoeI -
MJ69_RS07950 1322466..1323395 - 930 WP_000803351.1 LysR family transcriptional regulator -
MJ69_RS07955 1323441..1324265 - 825 WP_000754737.1 SDR family oxidoreductase -
MJ69_RS07960 1324348..1324602 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
MJ69_RS07965 1324599..1324850 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
MJ69_RS07970 1325133..1325180 + 48 WP_001375657.1 his operon leader peptide -
MJ69_RS07980 1325326..1326225 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
MJ69_RS07985 1326231..1327535 + 1305 WP_000009589.1 histidinol dehydrogenase -
MJ69_RS07990 1327532..1328602 + 1071 WP_032323947.1 histidinol-phosphate transaminase -
MJ69_RS07995 1328602..1329669 + 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98820 WP_000767829.1 NZ_CP027442:c1324602-1324348 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98820 NZ_CP027442:c1324602-1324348 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98820 WP_001259255.1 NZ_CP027442:c1324850-1324599 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98820 NZ_CP027442:c1324850-1324599 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References