Detailed information of TA system TA098803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3127031..3127633
• Replicon: chromosome
• Accession: NZ_CP027440
• Organism: Escherichia coli strain 2012C-4502

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6W69_RS16275
• Protein ID: WP_000897305.1
• Coordinates: 3127031..3127342 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6W69_RS16280
• Protein ID: WP_000356397.1
• Coordinates: 3127343..3127633 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W69_RS16250	3122945..3123544	+	600	WP_001298594.1	glucose-1-phosphatase	-
C6W69_RS16255	3123538..3124410	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C6W69_RS16260	3124407..3124844	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6W69_RS16265	3124889..3125830	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6W69_RS16270	3125894..3126802	-	909	WP_053290819.1	alpha/beta hydrolase	-
C6W69_RS16275	3127031..3127342	+	312	WP_000897305.1	hypothetical protein	Toxin
C6W69_RS16280	3127343..3127633	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6W69_RS16290	3128238..3128456	+	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
C6W69_RS16295	3128671..3129600	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6W69_RS16300	3129597..3130232	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6W69_RS16305	3130229..3131131	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98803 WP_000897305.1 NZ_CP027440:3127031-3127342 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98803 NZ_CP027440:3127031-3127342 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98803 WP_000356397.1 NZ_CP027440:3127343-3127633 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98803 NZ_CP027440:3127343-3127633 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7