Detailed information of TA system TA098801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2286600..2287399
• Replicon: chromosome
• Accession: NZ_CP027440
• Organism: Escherichia coli strain 2012C-4502

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: C6W69_RS11945
• Protein ID: WP_106905254.1
• Coordinates: 2286935..2287399 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: C6W69_RS11940
• Protein ID: WP_001296435.1
• Coordinates: 2286600..2286935 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W69_RS11925	2282385..2283155	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
C6W69_RS11930	2283171..2284505	-	1335	WP_106905253.1	galactarate/glucarate/glycerate transporter GarP	-
C6W69_RS11935	2284880..2286451	+	1572	WP_001273939.1	galactarate dehydratase	-
C6W69_RS11940	2286600..2286935	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
C6W69_RS11945	2286935..2287399	+	465	WP_106905254.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
C6W69_RS11950	2287454..2288263	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
C6W69_RS11960	2288512..2289792	+	1281	WP_000681938.1	tagatose-bisphosphate aldolase subunit KbaZ	-
C6W69_RS11965	2289815..2290288	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
C6W69_RS11970	2290299..2291078	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
C6W69_RS11975	2291068..2291946	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
C6W69_RS11980	2291964..2292398	+	435	WP_000948823.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2278904..2287399	8495

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17734.11 Da
• Isoelectric Point: 9.2153

>T98801 WP_106905254.1 NZ_CP027440:2286935-2287399 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSLVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T98801 NZ_CP027440:2286935-2287399 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACTGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT98801 WP_001296435.1 NZ_CP027440:2286600-2286935 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT98801 NZ_CP027440:2286600-2286935 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: S1PPV5