Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 506342..506904 Replicon chromosome
Accession NZ_CP027440
Organism Escherichia coli strain 2012C-4502

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag C6W69_RS02895 Protein ID WP_000605676.1
Coordinates 506626..506904 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag C6W69_RS02890 Protein ID WP_000781370.1
Coordinates 506342..506626 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W69_RS02875 501701..504748 + 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -
C6W69_RS02880 504761..505645 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
C6W69_RS02885 505638..506291 + 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
C6W69_RS02890 506342..506626 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
C6W69_RS02895 506626..506904 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C6W69_RS02900 507090..508100 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
C6W69_RS02905 508234..509931 - 1698 WP_001357345.1 malate dehydrogenase -
C6W69_RS02910 510088..510225 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
C6W69_RS02915 510327..510542 - 216 WP_000495766.1 biofilm-dependent modulation protein -
C6W69_RS02925 510887..511318 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T98789 WP_000605676.1 NZ_CP027440:c506904-506626 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T98789 NZ_CP027440:c506904-506626 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT98789 WP_000781370.1 NZ_CP027440:c506626-506342 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT98789 NZ_CP027440:c506626-506342 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCGGCGCTCACTCCGGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References