Detailed information of TA system TA098784

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 404600..404971
• Replicon: chromosome
• Accession: NZ_CP027440
• Organism: Escherichia coli strain 2012C-4502

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: C6W69_RS02400
• Protein ID: WP_001317028.1
• Coordinates: 404777..404971 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	404600..404778 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W69_RS02375	400555..401928	+	1374	WP_106905052.1	ATP-dependent RNA helicase DbpA	-
C6W69_RS02380	402057..402992	-	936	WP_001157377.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
C6W69_RS02385	403044..404279	-	1236	WP_000040839.1	site-specific integrase	-
C6W69_RS02390	404281..404496	-	216	WP_000079604.1	excisionase XisR	-
C6W69_RS02395	404596..404784	-	189	WP_001595656.1	DUF1187 family protein	-
-	404600..404778	+	179	NuclAT_0	-	Antitoxin
-	404600..404778	+	179	NuclAT_0	-	Antitoxin
-	404600..404778	+	179	NuclAT_0	-	Antitoxin
-	404600..404778	+	179	NuclAT_0	-	Antitoxin
C6W69_RS02400	404777..404971	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C6W69_RS02405	405028..405327	-	300	Protein_450	recombinase RecT	-
C6W69_RS02410	405328..405843	+	516	Protein_451	DUF3672 domain-containing protein	-
C6W69_RS02415	405910..406509	+	600	WP_001230359.1	Ail/Lom family outer membrane beta-barrel protein	-
C6W69_RS02420	406574..409879	+	3306	WP_072025145.1	prophage tail fiber N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	403044..409879	6835

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T98784 WP_001317028.1 NZ_CP027440:c404971-404777 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T98784 NZ_CP027440:c404971-404777 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT98784 NZ_CP027440:404600-404778 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTAGTTAGTTTATATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin