Detailed information of TA system TA098776

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 38953..39223
• Replicon: plasmid unnamed2
• Accession: NZ_CP027439
• Organism: Escherichia coli strain 2012C-4221

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C6W65_RS26240
• Protein ID: WP_001312861.1
• Coordinates: 39065..39223 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	38953..39016 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W65_RS26195	33965..34427	-	463	Protein_41	hypothetical protein	-
C6W65_RS27090	34497..34703	+	207	WP_000275848.1	hypothetical protein	-
C6W65_RS26210	34729..35262	+	534	WP_106907088.1	single-stranded DNA-binding protein	-
C6W65_RS26215	35325..35558	+	234	WP_021564200.1	DUF905 family protein	-
C6W65_RS26220	35622..37580	+	1959	WP_106907090.1	ParB/RepB/Spo0J family partition protein	-
C6W65_RS26225	37635..38069	+	435	WP_000845926.1	conjugation system SOS inhibitor PsiB	-
C6W65_RS26230	38066..38785	+	720	WP_106907092.1	plasmid SOS inhibition protein A	-
-	38797..39021	+	225	NuclAT_0	-	-
-	38797..39021	+	225	NuclAT_0	-	-
-	38797..39021	+	225	NuclAT_0	-	-
-	38797..39021	+	225	NuclAT_0	-	-
-	38953..39016	-	64	-	-	Antitoxin
C6W65_RS26240	39065..39223	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
C6W65_RS27095	39577..39789	-	213	WP_162491396.1	hypothetical protein	-
C6W65_RS26260	40138..40425	+	288	WP_000107538.1	hypothetical protein	-
C6W65_RS26265	40543..41364	+	822	WP_158709101.1	DUF945 domain-containing protein	-
C6W65_RS26270	41661..42251	-	591	WP_168926342.1	transglycosylase SLT domain-containing protein	-
C6W65_RS26275	42540..42923	+	384	WP_046788507.1	relaxosome protein TraM	-
C6W65_RS26280	43110..43799	+	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	acrB / vat	1..107188	107188

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T98776 WP_001312861.1 NZ_CP027439:39065-39223 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T98776 NZ_CP027439:39065-39223 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT98776 NZ_CP027439:c39016-38953 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin