Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4999041..4999543 Replicon chromosome
Accession NZ_CP027437
Organism Escherichia coli strain 2012C-4221

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6W65_RS25360 Protein ID WP_000767829.1
Coordinates 4999289..4999543 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6W65_RS25355 Protein ID WP_001259255.1
Coordinates 4999041..4999292 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W65_RS25325 4994219..4995286 - 1068 WP_106906896.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C6W65_RS25330 4995286..4996356 - 1071 WP_000108954.1 histidinol-phosphate transaminase -
C6W65_RS25335 4996353..4997657 - 1305 WP_001559366.1 histidinol dehydrogenase -
C6W65_RS25340 4997663..4998562 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6W65_RS25350 4998708..4998758 - 51 WP_001364200.1 his operon leader peptide -
C6W65_RS25355 4999041..4999292 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6W65_RS25360 4999289..4999543 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6W65_RS25365 4999626..5000450 + 825 WP_000754737.1 SDR family oxidoreductase -
C6W65_RS25370 5000496..5001425 + 930 WP_000803351.1 LysR family transcriptional regulator -
C6W65_RS25375 5001640..5001702 + 63 WP_010723108.1 membrane protein YoeI -
C6W65_RS25380 5001692..5003050 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98768 WP_000767829.1 NZ_CP027437:4999289-4999543 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98768 NZ_CP027437:4999289-4999543 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98768 WP_001259255.1 NZ_CP027437:4999041-4999292 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98768 NZ_CP027437:4999041-4999292 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCTGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References