Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4161352..4162079 Replicon chromosome
Accession NZ_CP027437
Organism Escherichia coli strain 2012C-4221

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag C6W65_RS21270 Protein ID WP_106906679.1
Coordinates 4161352..4161663 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C6W65_RS21275 Protein ID WP_106906680.1
Coordinates 4161660..4162079 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6W65_RS21245 4157287..4158996 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
C6W65_RS21250 4159006..4159548 + 543 WP_000493779.1 formate hydrogenlyase subunit HycF -
C6W65_RS21255 4159548..4160315 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
C6W65_RS21260 4160312..4160722 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
C6W65_RS21265 4160715..4161185 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
C6W65_RS21270 4161352..4161663 + 312 WP_106906679.1 type II toxin-antitoxin system HigB family toxin Toxin
C6W65_RS21275 4161660..4162079 + 420 WP_106906680.1 helix-turn-helix domain-containing protein Antitoxin
C6W65_RS21280 4162158..4163582 - 1425 WP_106906954.1 6-phospho-beta-glucosidase AscB -
C6W65_RS21285 4163591..4165048 - 1458 WP_106906681.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
C6W65_RS21290 4165308..4166318 + 1011 WP_024193122.1 DNA-binding transcriptional regulator AscG -
C6W65_RS21295 4166467..4166994 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12411.09 Da        Isoelectric Point: 9.5262

>T98767 WP_106906679.1 NZ_CP027437:4161352-4161663 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDAGGNNLRAIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T98767 NZ_CP027437:4161352-4161663 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGCCGGCGGGAACAATCTTAGGGCTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15402.39 Da        Isoelectric Point: 4.4596

>AT98767 WP_106906680.1 NZ_CP027437:4161660-4162079 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAGMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT98767 NZ_CP027437:4161660-4162079 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGTAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGGAATGCCGGTT
GGCGTGGCGTTACTTCGTACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References