Detailed information of TA system TA098764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3695818..3696617
• Replicon: chromosome
• Accession: NZ_CP027437
• Organism: Escherichia coli strain 2012C-4221

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: C6W65_RS18915
• Protein ID: WP_106906581.1
• Coordinates: 3695818..3696282 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A368IY36
• Locus tag: C6W65_RS18920
• Protein ID: WP_001604880.1
• Coordinates: 3696282..3696617 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W65_RS18880	3690819..3691253	-	435	WP_106906578.1	PTS sugar transporter subunit IIA	-
C6W65_RS18885	3691271..3692149	-	879	WP_106906579.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
C6W65_RS18890	3692139..3692918	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
C6W65_RS18895	3692929..3693402	-	474	WP_106906580.1	PTS N-acetylgalactosamine transporter subunit IIB	-
C6W65_RS18900	3693425..3694705	-	1281	WP_023908831.1	tagatose-bisphosphate aldolase subunit KbaZ	-
C6W65_RS18910	3694954..3695763	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
C6W65_RS18915	3695818..3696282	-	465	WP_106906581.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
C6W65_RS18920	3696282..3696617	-	336	WP_001604880.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
C6W65_RS18925	3696766..3698337	-	1572	WP_001273757.1	galactarate dehydratase	-
C6W65_RS18930	3698712..3700046	+	1335	WP_000599654.1	galactarate/glucarate/glycerate transporter GarP	-
C6W65_RS18935	3700062..3700832	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17848.31 Da
• Isoelectric Point: 9.6924

>T98764 WP_106906581.1 NZ_CP027437:c3696282-3695818 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEILKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T98764 NZ_CP027437:c3696282-3695818 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGATATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12340.91 Da
• Isoelectric Point: 4.8616

>AT98764 WP_001604880.1 NZ_CP027437:c3696617-3696282 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGLDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT98764 NZ_CP027437:c3696617-3696282 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACCATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAGCAAGGAAAGAAACTGGTCGCTGGCCTGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A368IY36