Detailed information of TA system TA098757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1969862..1970556
• Replicon: chromosome
• Accession: NZ_CP027437
• Organism: Escherichia coli strain 2012C-4221

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: C6W65_RS10225
• Protein ID: WP_001521903.1
• Coordinates: 1969862..1970260 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: C6W65_RS10230
• Protein ID: WP_000554758.1
• Coordinates: 1970263..1970556 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6W65_RS10195	1964946..1965347	-	402	WP_000174681.1	sigma factor-binding protein Crl	-
C6W65_RS10200	1965405..1966649	-	1245	WP_000189541.1	esterase FrsA	-
-	1966065..1966145	-	81	NuclAT_7	-	-
-	1966065..1966145	-	81	NuclAT_7	-	-
-	1966065..1966145	-	81	NuclAT_7	-	-
-	1966065..1966145	-	81	NuclAT_7	-	-
C6W65_RS10205	1966741..1967199	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
C6W65_RS10210	1967460..1968917	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
C6W65_RS10215	1968974..1969363	-	390	Protein_1879	peptide chain release factor H	-
C6W65_RS10220	1969400..1969852	-	453	WP_059342012.1	GNAT family N-acetyltransferase	-
C6W65_RS10225	1969862..1970260	-	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C6W65_RS10230	1970263..1970556	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C6W65_RS10235	1970608..1971663	-	1056	WP_034173577.1	DNA polymerase IV	-
C6W65_RS10240	1971734..1972519	-	786	WP_199851529.1	putative lateral flagellar export/assembly protein LafU	-
C6W65_RS10245	1972491..1974203	+	1713	Protein_1885	flagellar biosynthesis protein FlhA	-
C6W65_RS10250	1974365..1975114	-	750	WP_158709051.1	C40 family peptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	1931390..1970556	39166

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T98757 WP_001521903.1 NZ_CP027437:c1970260-1969862 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T98757 NZ_CP027437:c1970260-1969862 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT98757 WP_000554758.1 NZ_CP027437:c1970556-1970263 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT98757 NZ_CP027437:c1970556-1970263 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGTTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3