Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4787822..4788549 Replicon chromosome
Accession NZ_CP027435
Organism Escherichia coli strain 2014C-3599

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag ND02_RS25470 Protein ID WP_000550189.1
Coordinates 4788235..4788549 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag ND02_RS25465 Protein ID WP_000560266.1
Coordinates 4787822..4788238 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ND02_RS25455 4783082..4785433 + 2352 WP_000695479.1 alpha-glucosidase -
ND02_RS25460 4785759..4787777 + 2019 WP_106902991.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
ND02_RS25465 4787822..4788238 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
ND02_RS25470 4788235..4788549 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
ND02_RS25480 4788834..4789970 - 1137 WP_000018685.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
ND02_RS25485 4790055..4790558 + 504 WP_001295542.1 M48 family metallopeptidase -
ND02_RS25490 4790635..4791327 + 693 WP_000942583.1 vancomycin high temperature exclusion protein -
ND02_RS25495 4791406..4792392 + 987 WP_001297165.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T98741 WP_000550189.1 NZ_CP027435:c4788549-4788235 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T98741 NZ_CP027435:c4788549-4788235 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT98741 WP_000560266.1 NZ_CP027435:c4788238-4787822 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT98741 NZ_CP027435:c4788238-4787822 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCAAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References