Detailed information of TA system TA098735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4243034..4243761
• Replicon: chromosome
• Accession: NZ_CP027435
• Organism: Escherichia coli strain 2014C-3599

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: ND02_RS22550
• Protein ID: WP_000547564.1
• Coordinates: 4243450..4243761 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ND02_RS22545
• Protein ID: WP_000126294.1
• Coordinates: 4243034..4243453 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ND02_RS22525	4238164..4238691	-	528	WP_001078777.1	electron transport protein HydN	-
ND02_RS22530	4238840..4239850	-	1011	WP_001343660.1	DNA-binding transcriptional regulator AscG	-
ND02_RS22535	4240110..4241486	+	1377	WP_001107877.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
ND02_RS22540	4241495..4242919	+	1425	WP_000110319.1	6-phospho-beta-glucosidase AscB	-
ND02_RS22545	4243034..4243453	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
ND02_RS22550	4243450..4243761	-	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
ND02_RS22555	4243935..4244696	-	762	WP_001026445.1	hypothetical protein	-
ND02_RS22560	4244721..4245191	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
ND02_RS22565	4245184..4245594	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
ND02_RS22570	4245591..4246358	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
ND02_RS22575	4246358..4246900	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
ND02_RS22580	4246910..4248619	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T98735 WP_000547564.1 NZ_CP027435:c4243761-4243450 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T98735 NZ_CP027435:c4243761-4243450 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT98735 WP_000126294.1 NZ_CP027435:c4243453-4243034 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT98735 NZ_CP027435:c4243453-4243034 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAAAGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5