Detailed information of TA system TA098720

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2550983..2551354
• Replicon: chromosome
• Accession: NZ_CP027435
• Organism: Escherichia coli strain 2014C-3599

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: ND02_RS13595
• Protein ID: WP_001317028.1
• Coordinates: 2551160..2551354 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2550983..2551161 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ND02_RS13565	2546735..2546908	+	174	WP_001296046.1	hypothetical protein	-
ND02_RS13570	2546938..2548311	+	1374	WP_000123758.1	ATP-dependent RNA helicase DbpA	-
ND02_RS13575	2548440..2549375	-	936	WP_001157421.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
ND02_RS13580	2549427..2550662	-	1236	WP_000040852.1	site-specific integrase	-
ND02_RS13585	2550664..2550879	-	216	WP_000079604.1	excisionase XisR	-
ND02_RS13590	2550958..2551167	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	2550983..2551161	+	179	NuclAT_0	-	Antitoxin
-	2550983..2551161	+	179	NuclAT_0	-	Antitoxin
-	2550983..2551161	+	179	NuclAT_0	-	Antitoxin
-	2550983..2551161	+	179	NuclAT_0	-	Antitoxin
ND02_RS13595	2551160..2551354	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
ND02_RS13600	2551411..2552220	-	810	WP_000595429.1	recombination protein RecT	-
ND02_RS13605	2552213..2554882	-	2670	WP_000102191.1	exodeoxyribonuclease VIII	-
ND02_RS13610	2554963..2555139	-	177	WP_000245534.1	hypothetical protein	-
ND02_RS13615	2555133..2555354	-	222	WP_000560223.1	killing protein KilR	-
ND02_RS13620	2555779..2555931	-	153	WP_001169149.1	DUF1391 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	2549427..2597668	48241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T98720 WP_001317028.1 NZ_CP027435:c2551354-2551160 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T98720 NZ_CP027435:c2551354-2551160 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT98720 NZ_CP027435:2550983-2551161 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin