Detailed information of TA system TA098712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 226639..227241
• Replicon: chromosome
• Accession: NZ_CP027435
• Organism: Escherichia coli strain 2014C-3599

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: ND02_RS01140
• Protein ID: WP_000897305.1
• Coordinates: 226639..226950 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ND02_RS01145
• Protein ID: WP_000356397.1
• Coordinates: 226951..227241 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ND02_RS01110	221683..222120	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
ND02_RS01115	222165..223106	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
ND02_RS01120	223470..224408	+	939	Protein_202	hypothetical protein	-
ND02_RS01125	224421..225335	+	915	WP_072097029.1	transposase	-
ND02_RS01135	225503..226410	-	908	Protein_204	alpha/beta hydrolase	-
ND02_RS01140	226639..226950	+	312	WP_000897305.1	hypothetical protein	Toxin
ND02_RS01145	226951..227241	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
ND02_RS01155	227827..228045	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
ND02_RS01160	228264..228506	+	243	WP_001086388.1	hypothetical protein	-
ND02_RS01165	228836..229765	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
ND02_RS01170	229762..230397	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
ND02_RS01175	230394..231296	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98712 WP_000897305.1 NZ_CP027435:226639-226950 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98712 NZ_CP027435:226639-226950 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98712 WP_000356397.1 NZ_CP027435:226951-227241 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98712 NZ_CP027435:226951-227241 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7