Detailed information of TA system TA098691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1243359..1244053
• Replicon: chromosome
• Accession: NZ_CP027430
• Organism: Escherichia coli strain AS19-RrmA-

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: C6P57_RS06655
• Protein ID: WP_001263493.1
• Coordinates: 1243359..1243757 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: C6P57_RS06660
• Protein ID: WP_000554757.1
• Coordinates: 1243760..1244053 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P57_RS06625	1238359..1239603	-	1245	WP_000189541.1	esterase FrsA	-
-	1239019..1239099	-	81	NuclAT_11	-	-
-	1239019..1239099	-	81	NuclAT_11	-	-
-	1239019..1239099	-	81	NuclAT_11	-	-
-	1239019..1239099	-	81	NuclAT_11	-	-
C6P57_RS06630	1239695..1240153	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
C6P57_RS06635	1240414..1241871	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
C6P57_RS06640	1241928..1242449	-	522	Protein_1206	peptide chain release factor H	-
C6P57_RS06645	1242448..1242651	-	204	Protein_1207	RNA ligase RtcB family protein	-
C6P57_RS06650	1242897..1243349	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
C6P57_RS06655	1243359..1243757	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C6P57_RS06660	1243760..1244053	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C6P57_RS06665	1244105..1245160	-	1056	WP_001226164.1	DNA polymerase IV	-
C6P57_RS06670	1245231..1246016	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
C6P57_RS06675	1245988..1247700	+	1713	Protein_1213	flagellar biosynthesis protein FlhA	-
C6P57_RS06680	1247916..1248413	-	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	gmhA/lpcA	1237089..1276176	39087

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T98691 WP_001263493.1 NZ_CP027430:c1243757-1243359 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T98691 NZ_CP027430:c1243757-1243359 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT98691 WP_000554757.1 NZ_CP027430:c1244053-1243760 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT98691 NZ_CP027430:c1244053-1243760 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6