Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 920898..921465 Replicon chromosome
Accession NZ_CP027417
Organism Oligella urethralis strain FDAARGOS_329

Toxin (Protein)


Gene name higB Uniprot ID A0A2X1UM99
Locus tag CEQ07_RS04285 Protein ID WP_048769327.1
Coordinates 920898..921176 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2N6QF66
Locus tag CEQ07_RS04290 Protein ID WP_018027458.1
Coordinates 921187..921465 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ07_RS04260 916899..917519 + 621 WP_105885214.1 XRE family transcriptional regulator -
CEQ07_RS04265 917541..918836 - 1296 WP_105885215.1 MHS family MFS transporter -
CEQ07_RS04270 919041..919658 + 618 WP_105885216.1 transposase -
CEQ07_RS04275 919775..920308 - 534 WP_070476880.1 hypothetical protein -
CEQ07_RS04280 920307..920732 + 426 WP_151457947.1 integrase core domain-containing protein -
CEQ07_RS04285 920898..921176 + 279 WP_048769327.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CEQ07_RS04290 921187..921465 + 279 WP_018027458.1 HigA family addiction module antidote protein Antitoxin
CEQ07_RS04295 921568..921798 + 231 WP_070477506.1 TraY domain-containing protein -
CEQ07_RS04300 921847..922299 + 453 WP_105885217.1 transposase -
CEQ07_RS04305 922572..924128 + 1557 WP_070476221.1 autotransporter outer membrane beta-barrel domain-containing protein -
CEQ07_RS04310 924159..924677 + 519 WP_105885219.1 OmpA family protein -
CEQ07_RS04315 924933..925943 - 1011 WP_105885018.1 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 906121..934595 28474
- flank IS/Tn - - 924933..925943 1010


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(3-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(10-59)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10804.29 Da        Isoelectric Point: 9.9606

>T98664 WP_048769327.1 NZ_CP027417:920898-921176 [Oligella urethralis]
MIKSFKHKGLRKYFETGNTSGIQSKHAVRLRTQLSVLDTIKSPDEINIVSWRLHALTGNLKGYWSITVNANWRITFRFEG
ENVYEVDYLDYH

Download         Length: 279 bp

>T98664 NZ_CP027417:920898-921176 [Oligella urethralis]
ATGATTAAATCATTTAAACATAAAGGCCTTAGAAAGTATTTTGAAACTGGAAATACTTCAGGTATTCAATCAAAACATGC
AGTTAGGTTACGTACCCAGCTTTCAGTATTAGATACGATTAAAAGCCCTGATGAAATTAATATAGTTAGTTGGAGATTGC
ATGCTTTGACTGGTAATTTAAAAGGCTACTGGTCTATAACAGTTAATGCAAATTGGCGTATCACTTTTAGATTTGAAGGT
GAAAATGTTTATGAAGTTGATTACTTAGATTATCACTAG

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10359.88 Da        Isoelectric Point: 5.0348

>AT98664 WP_018027458.1 NZ_CP027417:921187-921465 [Oligella urethralis]
MRMYNPPHPGLVIREYLDDVTVSDAAKHLGITRTSLSRILNGKTGISPLMAIKLEKAFGGITAESWLAMQAKYDLWQEEQ
SYDDTVIPFEFA

Download         Length: 279 bp

>AT98664 NZ_CP027417:921187-921465 [Oligella urethralis]
ATGAGAATGTACAATCCACCTCACCCAGGTCTTGTTATTAGAGAGTATTTAGATGATGTTACTGTTTCAGATGCAGCAAA
ACATTTAGGCATTACTAGGACTTCTTTATCAAGAATACTCAATGGCAAAACAGGTATATCACCTTTAATGGCTATTAAAC
TTGAAAAAGCATTTGGGGGAATAACAGCTGAGTCTTGGTTAGCGATGCAAGCAAAATATGACTTATGGCAAGAAGAGCAA
AGTTACGATGATACAGTTATACCATTTGAATTTGCTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2X1UM99

Antitoxin

Source ID Structure
AlphaFold DB A0A2N6QF66

References