Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 87584..87848 Replicon plasmid unnamed1
Accession NZ_CP027395
Organism Escherichia coli O104:H4 strain FDAARGOS_349

Toxin (Protein)


Gene name pndA Uniprot ID U9Z417
Locus tag CEQ27_RS29015 Protein ID WP_001387489.1
Coordinates 87696..87848 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 87584..87646 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ27_RS29000 83686..84756 - 1071 WP_000151588.1 IncI1-type conjugal transfer protein TrbB -
CEQ27_RS29005 84775..85983 - 1209 WP_000121263.1 IncI1-type conjugal transfer protein TrbA -
CEQ27_RS29010 86290..87381 - 1092 WP_000426061.1 hypothetical protein -
- 87584..87646 - 63 NuclAT_0 - Antitoxin
- 87584..87646 - 63 NuclAT_0 - Antitoxin
- 87584..87646 - 63 NuclAT_0 - Antitoxin
- 87584..87646 - 63 NuclAT_0 - Antitoxin
CEQ27_RS29015 87696..87848 + 153 WP_001387489.1 Hok/Gef family protein Toxin
CEQ27_RS29020 87920..88171 - 252 WP_001291964.1 hypothetical protein -
CEQ27_RS29025 88471..88767 + 297 WP_001275298.1 hypothetical protein -
CEQ27_RS30085 88832..89008 - 177 WP_001054898.1 hypothetical protein -
CEQ27_RS29030 89188..89397 - 210 WP_001140543.1 hemolysin expression modulator Hha -
CEQ27_RS29035 89495..90109 - 615 WP_000578648.1 plasmid IncI1-type surface exclusion protein ExcA -
CEQ27_RS29040 90185..92352 - 2168 Protein_97 IncI1-type conjugal transfer membrane protein TraY -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-15 / blaTEM-1B - 1..117229 117229


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5761.10 Da        Isoelectric Point: 8.7948

>T98625 WP_001387489.1 NZ_CP027395:87696-87848 [Escherichia coli O104:H4]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T98625 NZ_CP027395:87696-87848 [Escherichia coli O104:H4]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 63 bp

>AT98625 NZ_CP027395:c87646-87584 [Escherichia coli O104:H4]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9Z417

Antitoxin

Download structure file

References