Detailed information of TA system TA098617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3241913..3242515
• Replicon: chromosome
• Accession: NZ_CP027394
• Organism: Escherichia coli O104:H4 strain FDAARGOS_349

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CEQ27_RS17850
• Protein ID: WP_000897305.1
• Coordinates: 3241913..3242224 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CEQ27_RS17855
• Protein ID: WP_000356397.1
• Coordinates: 3242225..3242515 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEQ27_RS17825	3237827..3238426	+	600	WP_001295269.1	glucose-1-phosphatase	-
CEQ27_RS17830	3238420..3239292	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CEQ27_RS17835	3239289..3239726	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CEQ27_RS17840	3239771..3240712	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CEQ27_RS17845	3240776..3241684	-	909	WP_001387201.1	alpha/beta hydrolase	-
CEQ27_RS17850	3241913..3242224	+	312	WP_000897305.1	hypothetical protein	Toxin
CEQ27_RS17855	3242225..3242515	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CEQ27_RS17865	3243101..3243319	+	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
CEQ27_RS17870	3243539..3243781	+	243	WP_001087409.1	hypothetical protein	-
CEQ27_RS17875	3244111..3245040	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CEQ27_RS17880	3245037..3245672	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CEQ27_RS17885	3245669..3246571	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98617 WP_000897305.1 NZ_CP027394:3241913-3242224 [Escherichia coli O104:H4]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98617 NZ_CP027394:3241913-3242224 [Escherichia coli O104:H4]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98617 WP_000356397.1 NZ_CP027394:3242225-3242515 [Escherichia coli O104:H4]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98617 NZ_CP027394:3242225-3242515 [Escherichia coli O104:H4]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTCAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7