Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1071486..1071988 Replicon chromosome
Accession NZ_CP027394
Organism Escherichia coli O104:H4 strain FDAARGOS_349

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CEQ27_RS06655 Protein ID WP_000767829.1
Coordinates 1071486..1071740 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CEQ27_RS06660 Protein ID WP_001259255.1
Coordinates 1071737..1071988 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEQ27_RS06625 1066501..1066728 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CEQ27_RS06630 1066742..1067800 - 1059 WP_000492324.1 transport protein YeeE -
CEQ27_RS06635 1067979..1069337 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CEQ27_RS06640 1069327..1069389 - 63 WP_010723108.1 membrane protein YoeI -
CEQ27_RS06645 1069604..1070533 - 930 WP_000803366.1 LysR family transcriptional regulator -
CEQ27_RS06650 1070579..1071403 - 825 WP_000754758.1 SDR family oxidoreductase -
CEQ27_RS06655 1071486..1071740 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CEQ27_RS06660 1071737..1071988 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CEQ27_RS06665 1072272..1072322 + 51 WP_001364200.1 his operon leader peptide -
CEQ27_RS06675 1072468..1073367 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CEQ27_RS06680 1073373..1074677 + 1305 WP_001386882.1 histidinol dehydrogenase -
CEQ27_RS06685 1074674..1075744 + 1071 WP_000108967.1 histidinol-phosphate transaminase -
CEQ27_RS06690 1075744..1076811 + 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98610 WP_000767829.1 NZ_CP027394:c1071740-1071486 [Escherichia coli O104:H4]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98610 NZ_CP027394:c1071740-1071486 [Escherichia coli O104:H4]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98610 WP_001259255.1 NZ_CP027394:c1071988-1071737 [Escherichia coli O104:H4]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98610 NZ_CP027394:c1071988-1071737 [Escherichia coli O104:H4]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References