Detailed information of TA system TA098595

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5151567..5152294
• Replicon: chromosome
• Accession: NZ_CP027390
• Organism: Escherichia coli strain 2015C-4944

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: AX062_RS28090
• Protein ID: WP_000547559.1
• Coordinates: 5151567..5151878 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AX062_RS28095
• Protein ID: WP_000126294.1
• Coordinates: 5151875..5152294 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AX062_RS28060	5146709..5148418	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
AX062_RS28065	5148428..5148970	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
AX062_RS28070	5148970..5149737	+	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
AX062_RS28075	5149734..5150144	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
AX062_RS28080	5150137..5150607	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
AX062_RS28085	5150650..5151393	+	744	WP_001341829.1	hypothetical protein	-
AX062_RS28090	5151567..5151878	+	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
AX062_RS28095	5151875..5152294	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
AX062_RS28100	5152408..5153832	-	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
AX062_RS28105	5153841..5155298	-	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
AX062_RS28110	5155558..5156568	+	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
AX062_RS28115	5156717..5157244	+	528	WP_001078781.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T98595 WP_000547559.1 NZ_CP027390:5151567-5151878 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T98595 NZ_CP027390:5151567-5151878 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT98595 WP_000126294.1 NZ_CP027390:5151875-5152294 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT98595 NZ_CP027390:5151875-5152294 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163