Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 332457..332959 Replicon chromosome
Accession NZ_CP027390
Organism Escherichia coli strain 2015C-4944

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AX062_RS01755 Protein ID WP_000767829.1
Coordinates 332705..332959 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AX062_RS01750 Protein ID WP_001259255.1
Coordinates 332457..332708 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AX062_RS01720 327638..328705 - 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AX062_RS01725 328705..329775 - 1071 WP_000108914.1 histidinol-phosphate transaminase -
AX062_RS01730 329772..331076 - 1305 WP_000009589.1 histidinol dehydrogenase -
AX062_RS01735 331082..331981 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
AX062_RS01745 332127..332174 - 48 WP_001375657.1 his operon leader peptide -
AX062_RS01750 332457..332708 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AX062_RS01755 332705..332959 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AX062_RS01760 333042..333866 + 825 WP_000754737.1 SDR family oxidoreductase -
AX062_RS01765 333912..334841 + 930 WP_000803351.1 LysR family transcriptional regulator -
AX062_RS01770 335056..335118 + 63 WP_010723108.1 membrane protein YoeI -
AX062_RS01775 335108..336466 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AX062_RS01780 336645..337703 + 1059 WP_000492324.1 transport protein YeeE -
AX062_RS01785 337717..337944 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98559 WP_000767829.1 NZ_CP027390:332705-332959 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98559 NZ_CP027390:332705-332959 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98559 WP_001259255.1 NZ_CP027390:332457-332708 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98559 NZ_CP027390:332457-332708 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References