Detailed information of TA system TA098552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3690474..3691076
• Replicon: chromosome
• Accession: NZ_CP027388
• Organism: Escherichia coli strain 2011C-4251

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6P70_RS20010
• Protein ID: WP_000897305.1
• Coordinates: 3690765..3691076 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6P70_RS20005
• Protein ID: WP_000356397.1
• Coordinates: 3690474..3690764 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P70_RS19975	3685549..3687156	-	1608	WP_001222883.1	dipeptide ABC transporter substrate-binding protein DppA	-
C6P70_RS19980	3687799..3688008	-	210	Protein_3637	hypothetical protein	-
C6P70_RS19985	3688000..3688980	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
C6P70_RS19990	3689209..3689451	-	243	WP_001086388.1	hypothetical protein	-
C6P70_RS19995	3689670..3689888	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6P70_RS20005	3690474..3690764	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6P70_RS20010	3690765..3691076	-	312	WP_000897305.1	hypothetical protein	Toxin
C6P70_RS20015	3691305..3692213	+	909	WP_001162704.1	alpha/beta hydrolase	-
C6P70_RS20020	3692277..3693218	-	942	WP_001300148.1	fatty acid biosynthesis protein FabY	-
C6P70_RS20025	3693263..3693700	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6P70_RS20030	3693697..3694569	-	873	WP_000920762.1	virulence factor BrkB family protein	-
C6P70_RS20035	3694563..3695162	-	600	WP_001295269.1	glucose-1-phosphatase	-
C6P70_RS20040	3695261..3696046	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3688000..3688980	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98552 WP_000897305.1 NZ_CP027388:c3691076-3690765 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98552 NZ_CP027388:c3691076-3690765 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98552 WP_000356397.1 NZ_CP027388:c3690764-3690474 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98552 NZ_CP027388:c3690764-3690474 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7