Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4015957..4016459 Replicon chromosome
Accession NZ_CP027387
Organism Escherichia coli strain 2014C-3057

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6P73_RS21255 Protein ID WP_000767829.1
Coordinates 4016205..4016459 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6P73_RS21250 Protein ID WP_001259255.1
Coordinates 4015957..4016208 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P73_RS21220 4011138..4012205 - 1068 WP_000080089.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C6P73_RS21225 4012205..4013275 - 1071 WP_000108914.1 histidinol-phosphate transaminase -
C6P73_RS21230 4013272..4014576 - 1305 WP_000009589.1 histidinol dehydrogenase -
C6P73_RS21235 4014582..4015481 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6P73_RS21245 4015627..4015674 - 48 WP_001375657.1 his operon leader peptide -
C6P73_RS21250 4015957..4016208 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6P73_RS21255 4016205..4016459 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6P73_RS21260 4016542..4017366 + 825 WP_000754737.1 SDR family oxidoreductase -
C6P73_RS21265 4017412..4018341 + 930 WP_000803351.1 LysR family transcriptional regulator -
C6P73_RS21270 4018556..4018618 + 63 WP_010723108.1 membrane protein YoeI -
C6P73_RS21275 4018608..4019966 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6P73_RS21280 4020145..4021203 + 1059 WP_000492324.1 transport protein YeeE -
C6P73_RS21285 4021217..4021444 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98503 WP_000767829.1 NZ_CP027387:4016205-4016459 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98503 NZ_CP027387:4016205-4016459 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98503 WP_001259255.1 NZ_CP027387:4015957-4016208 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98503 NZ_CP027387:4015957-4016208 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References