Detailed information of TA system TA098486

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19104..19530
• Replicon: plasmid unnamed4
• Accession: NZ_CP027384
• Organism: Escherichia coli strain 2013C-3250

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: AYN27_RS29865
• Protein ID: WP_001312861.1
• Coordinates: 19104..19262 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	19306..19530 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYN27_RS29825	14472..15163	-	692	Protein_16	conjugal transfer transcriptional regulator TraJ	-
AYN27_RS29830	15350..15733	-	384	WP_001151524.1	relaxosome protein TraM	-
AYN27_RS29835	16147..16656	+	510	Protein_18	transglycosylase SLT domain-containing protein	-
AYN27_RS29840	16953..17774	-	822	WP_001234469.1	DUF945 domain-containing protein	-
AYN27_RS29845	17895..18182	-	288	WP_000107535.1	hypothetical protein	-
AYN27_RS31495	18526..18750	+	225	WP_001427866.1	hypothetical protein	-
AYN27_RS29865	19104..19262	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	19306..19530	-	225	NuclAT_0	-	Antitoxin
-	19306..19530	-	225	NuclAT_0	-	Antitoxin
-	19306..19530	-	225	NuclAT_0	-	Antitoxin
-	19306..19530	-	225	NuclAT_0	-	Antitoxin
AYN27_RS31500	19342..19530	+	189	WP_001299721.1	hypothetical protein	-
AYN27_RS29870	19499..20263	-	765	Protein_24	plasmid SOS inhibition protein A	-
AYN27_RS29875	20260..20695	-	436	Protein_25	conjugation system SOS inhibitor PsiB	-
AYN27_RS29880	20750..22710	-	1961	Protein_26	ParB/RepB/Spo0J family partition protein	-
AYN27_RS29885	22769..23002	-	234	WP_000006004.1	DUF905 domain-containing protein	-
AYN27_RS29895	23128..23586	-	459	WP_106914166.1	single-stranded DNA-binding protein	-
AYN27_RS29910	24232..24482	-	251	Protein_29	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..27224	27224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T98486 WP_001312861.1 NZ_CP027384:c19262-19104 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T98486 NZ_CP027384:c19262-19104 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT98486 NZ_CP027384:c19530-19306 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin