Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1764523..1765025 Replicon chromosome
Accession NZ_CP027380
Organism Escherichia coli strain 2013C-3250

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag AYN27_RS09545 Protein ID WP_000767829.1
Coordinates 1764771..1765025 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag AYN27_RS09540 Protein ID WP_001259255.1
Coordinates 1764523..1764774 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYN27_RS09510 1759694..1760761 - 1068 WP_000080054.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
AYN27_RS09515 1760758..1761837 - 1080 WP_000102292.1 histidinol-phosphate transaminase -
AYN27_RS09520 1761834..1763138 - 1305 WP_001345258.1 histidinol dehydrogenase -
AYN27_RS09525 1763144..1764043 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
AYN27_RS09535 1764189..1764239 - 51 WP_001364200.1 his operon leader peptide -
AYN27_RS09540 1764523..1764774 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
AYN27_RS09545 1764771..1765025 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
AYN27_RS09550 1765108..1765932 + 825 WP_000754755.1 SDR family oxidoreductase -
AYN27_RS09555 1765978..1766907 + 930 WP_000803331.1 LysR family transcriptional regulator -
AYN27_RS09560 1767122..1767184 + 63 WP_010723108.1 membrane protein YoeI -
AYN27_RS09565 1767174..1768532 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
AYN27_RS09570 1768711..1769769 + 1059 WP_000492340.1 transport protein YeeE -
AYN27_RS09575 1769783..1770010 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98466 WP_000767829.1 NZ_CP027380:1764771-1765025 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98466 NZ_CP027380:1764771-1765025 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98466 WP_001259255.1 NZ_CP027380:1764523-1764774 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98466 NZ_CP027380:1764523-1764774 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References