Detailed information of TA system TA098454

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 21811..22228
• Replicon: plasmid unnamed2
• Accession: NZ_CP027375
• Organism: Escherichia coli strain 05-3629

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: C6P66_RS26205
• Protein ID: WP_000775238.1
• Coordinates: 21811..21972 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	22037..22228 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P66_RS26160	17623..18006	-	384	WP_001585112.1	relaxosome protein TraM	-
C6P66_RS26165	18327..18929	+	603	WP_106916400.1	transglycosylase SLT domain-containing protein	-
C6P66_RS26170	19225..20046	-	822	WP_024213651.1	DUF945 domain-containing protein	-
C6P66_RS26175	20165..20452	-	288	WP_000107542.1	hypothetical protein	-
C6P66_RS26185	20608..20910	-	303	WP_024213649.1	hypothetical protein	-
C6P66_RS27185	21213..21510	+	298	Protein_30	hypothetical protein	-
C6P66_RS26205	21811..21972	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	22037..22228	+	192	-	-	Antitoxin
-	22037..22231	-	195	NuclAT_0	-	-
-	22037..22231	-	195	NuclAT_0	-	-
-	22037..22231	-	195	NuclAT_0	-	-
-	22037..22231	-	195	NuclAT_0	-	-
C6P66_RS26210	22180..22494	-	315	Protein_32	theronine dehydrogenase	-
C6P66_RS26215	22491..23210	-	720	WP_001276237.1	plasmid SOS inhibition protein A	-
C6P66_RS26220	23207..23641	-	435	WP_024213646.1	conjugation system SOS inhibitor PsiB	-
C6P66_RS26225	23696..25654	-	1959	WP_052892458.1	ParB/RepB/Spo0J family partition protein	-
C6P66_RS26230	25720..25953	-	234	WP_021564200.1	DUF905 family protein	-
C6P66_RS26235	26011..26544	-	534	WP_024213643.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..118863	118863

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T98454 WP_000775238.1 NZ_CP027375:c21972-21811 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T98454 NZ_CP027375:c21972-21811 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 192 bp

>AT98454 NZ_CP027375:22037-22228 [Escherichia coli]
GCCTCGTGGGTTAATGAAAAATTAACTACGGGGCTTTTGTCCTTCTGCCACACAACACGGTAACAAACCACCTTCACGTC
ATGAGGCAGAAAGCCTCAAGCTCCGGGCACATCATAGCCCATATACCAGCACGCTGACCACACTCACTTTCCCTGAAAAT
AATCCGGTCGTTCAGCCAGTTCACGGGCAATC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin