Detailed information of TA system TA098449

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 13455..13719
• Replicon: plasmid unnamed1
• Accession: NZ_CP027374
• Organism: Escherichia coli strain 05-3629

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: C6P66_RS25435
• Protein ID: WP_001303307.1
• Coordinates: 13567..13719 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	13455..13517 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P66_RS25420	8694..10985	-	2292	WP_106916374.1	conjugal transfer protein TrbC	-
C6P66_RS25425	10978..12048	-	1071	WP_052895276.1	IncI1-type conjugal transfer protein TrbB	-
C6P66_RS25430	12067..13275	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	13455..13517	-	63	NuclAT_0	-	Antitoxin
-	13455..13517	-	63	NuclAT_0	-	Antitoxin
-	13455..13517	-	63	NuclAT_0	-	Antitoxin
-	13455..13517	-	63	NuclAT_0	-	Antitoxin
C6P66_RS25435	13567..13719	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
C6P66_RS25440	13791..14042	-	252	WP_001291965.1	hypothetical protein	-
-	14429..14480	-	52	NuclAT_1	-	-
-	14429..14480	-	52	NuclAT_1	-	-
-	14429..14480	-	52	NuclAT_1	-	-
-	14429..14480	-	52	NuclAT_1	-	-
C6P66_RS27175	15320..15457	-	138	WP_049290858.1	hypothetical protein	-
C6P66_RS25460	15502..16728	-	1227	WP_052895285.1	transposase	-
C6P66_RS25465	17331..17540	+	210	WP_052895278.1	HEAT repeat domain-containing protein	-
C6P66_RS25470	17612..18274	-	663	WP_052895279.1	plasmid IncI1-type surface exclusion protein ExcA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..91648	91648
	-	flank	IS/Tn	-	-	15502..16734	1232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T98449 WP_001303307.1 NZ_CP027374:13567-13719 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T98449 NZ_CP027374:13567-13719 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT98449 NZ_CP027374:c13517-13455 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin