98432

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 1887633..1888235 Replicon chromosome
Accession NZ_CP027373
Organism Escherichia coli strain 05-3629

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag C6P66_RS09800 Protein ID WP_000897305.1
Coordinates 1887924..1888235 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag C6P66_RS09795 Protein ID WP_000356397.1
Coordinates 1887633..1887923 (-) Length 97 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P66_RS09765 1883559..1884461 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
C6P66_RS09770 1884458..1885093 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
C6P66_RS09775 1885090..1886019 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
C6P66_RS09780 1886349..1886591 - 243 WP_001086388.1 hypothetical protein -
C6P66_RS09785 1886810..1887028 - 219 WP_001297075.1 ribbon-helix-helix domain-containing protein -
C6P66_RS09795 1887633..1887923 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
C6P66_RS09800 1887924..1888235 - 312 WP_000897305.1 hypothetical protein Toxin
C6P66_RS09805 1888464..1889372 + 909 WP_001162704.1 alpha/beta hydrolase -
C6P66_RS09810 1889436..1890377 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
C6P66_RS09815 1890422..1890859 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
C6P66_RS09820 1890856..1891728 - 873 WP_000920762.1 virulence factor BrkB family protein -
C6P66_RS09825 1891722..1892321 - 600 WP_001295269.1 glucose-1-phosphatase -
C6P66_RS09830 1892420..1893205 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

(36-85)


Sequences


Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T98432 WP_000897305.1 NZ_CP027373:c1888235-1887924 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T98432 NZ_CP027373:c1888235-1887924 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT98432 WP_000356397.1 NZ_CP027373:c1887923-1887633 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT98432 NZ_CP027373:c1887923-1887633 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7


Antitoxin

Source ID Structure

References