Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1076490..1077031 Replicon chromosome
Accession NZ_CP027373
Organism Escherichia coli strain 05-3629

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag C6P66_RS05780 Protein ID WP_000615976.1
Coordinates 1076753..1077031 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag C6P66_RS05775 Protein ID WP_000729704.1
Coordinates 1076490..1076750 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P66_RS05750 1071526..1072665 + 1140 WP_097447642.1 flagellar type III secretion system protein FlhB -
C6P66_RS05755 1072649..1074742 + 2094 WP_001583165.1 flagellar biosynthesis protein FlhA -
C6P66_RS05760 1074847..1075125 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
C6P66_RS05765 1075118..1075474 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
C6P66_RS05770 1075531..1076304 - 774 WP_000543899.1 C40 family peptidase -
C6P66_RS05775 1076490..1076750 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
C6P66_RS05780 1076753..1077031 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
C6P66_RS05785 1077187..1077927 + 741 WP_001225679.1 murein L,D-transpeptidase -
C6P66_RS05790 1077898..1078665 - 768 WP_000333380.1 class II glutamine amidotransferase -
C6P66_RS05795 1078871..1079449 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T98428 WP_000615976.1 NZ_CP027373:1076753-1077031 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T98428 NZ_CP027373:1076753-1077031 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT98428 WP_000729704.1 NZ_CP027373:1076490-1076750 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT98428 NZ_CP027373:1076490-1076750 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References