Detailed information of TA system TA098421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4228864..4229466
• Replicon: chromosome
• Accession: NZ_CP027371
• Organism: Escherichia coli strain 2015C-3905

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6P67_RS22030
• Protein ID: WP_000897305.1
• Coordinates: 4228864..4229175 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6P67_RS22035
• Protein ID: WP_000356397.1
• Coordinates: 4229176..4229466 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P67_RS22000	4223894..4224679	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
C6P67_RS22005	4224778..4225377	+	600	WP_001315111.1	glucose-1-phosphatase	-
C6P67_RS22010	4225371..4226243	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C6P67_RS22015	4226240..4226677	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6P67_RS22020	4226722..4227663	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6P67_RS22025	4227727..4228635	-	909	WP_001162704.1	alpha/beta hydrolase	-
C6P67_RS22030	4228864..4229175	+	312	WP_000897305.1	hypothetical protein	Toxin
C6P67_RS22035	4229176..4229466	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6P67_RS22045	4230071..4230289	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6P67_RS22050	4230508..4230750	+	243	WP_001086388.1	hypothetical protein	-
C6P67_RS22055	4231080..4232009	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6P67_RS22060	4232006..4232641	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6P67_RS22065	4232638..4233540	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98421 WP_000897305.1 NZ_CP027371:4228864-4229175 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98421 NZ_CP027371:4228864-4229175 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98421 WP_000356397.1 NZ_CP027371:4229176-4229466 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98421 NZ_CP027371:4229176-4229466 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7