Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2169652..2170154 Replicon chromosome
Accession NZ_CP027371
Organism Escherichia coli strain 2015C-3905

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C6P67_RS11655 Protein ID WP_000767829.1
Coordinates 2169652..2169906 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C6P67_RS11660 Protein ID WP_001259255.1
Coordinates 2169903..2170154 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6P67_RS11625 2164667..2164894 - 228 WP_000985280.1 sulfurtransferase TusA family protein -
C6P67_RS11630 2164908..2165966 - 1059 WP_000492318.1 transport protein YeeE -
C6P67_RS11635 2166145..2167503 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C6P67_RS11640 2167493..2167555 - 63 WP_010723108.1 membrane protein YoeI -
C6P67_RS11645 2167770..2168699 - 930 WP_106879140.1 LysR family transcriptional regulator -
C6P67_RS11650 2168745..2169569 - 825 WP_000754758.1 SDR family oxidoreductase -
C6P67_RS11655 2169652..2169906 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C6P67_RS11660 2169903..2170154 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C6P67_RS11665 2170437..2170487 + 51 WP_001364200.1 his operon leader peptide -
C6P67_RS11675 2170633..2171532 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C6P67_RS11680 2171538..2172842 + 1305 WP_001356062.1 histidinol dehydrogenase -
C6P67_RS11685 2172839..2173909 + 1071 WP_106879141.1 histidinol-phosphate transaminase -
C6P67_RS11690 2173909..2174976 + 1068 WP_000080130.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T98414 WP_000767829.1 NZ_CP027371:c2169906-2169652 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T98414 NZ_CP027371:c2169906-2169652 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT98414 WP_001259255.1 NZ_CP027371:c2170154-2169903 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT98414 NZ_CP027371:c2170154-2169903 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References