Detailed information of TA system TA098373

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 98683..99103
• Replicon: plasmid unnamed
• Accession: NZ_CP027367
• Organism: Escherichia coli strain 89-3156

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: C6P65_RS26930
• Protein ID: WP_000775238.1
• Coordinates: 98683..98844 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	98890..99103 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P65_RS26890	94469..94852	-	384	WP_001151535.1	relaxosome protein TraM	-
C6P65_RS26895	95128..95775	+	648	WP_000602507.1	transglycosylase SLT domain-containing protein	-
C6P65_RS26900	96071..96892	-	822	WP_001234499.1	DUF945 domain-containing protein	-
C6P65_RS26905	97011..97298	-	288	WP_000107543.1	hypothetical protein	-
C6P65_RS26915	97455..97757	-	303	WP_001272236.1	hypothetical protein	-
C6P65_RS26930	98683..98844	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	98890..99103	-	214	NuclAT_0	-	Antitoxin
-	98890..99103	-	214	NuclAT_0	-	Antitoxin
-	98890..99103	-	214	NuclAT_0	-	Antitoxin
-	98890..99103	-	214	NuclAT_0	-	Antitoxin
C6P65_RS26935	99052..99366	-	315	WP_001562885.1	hypothetical protein	-
C6P65_RS26940	99363..100082	-	720	WP_001276237.1	plasmid SOS inhibition protein A	-
C6P65_RS26945	100079..100513	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
C6P65_RS26950	100568..102526	-	1959	WP_000117328.1	ParB/RepB/Spo0J family partition protein	-
C6P65_RS26955	102587..102820	-	234	WP_000005993.1	DUF905 family protein	-
C6P65_RS26960	102878..103399	-	522	WP_000290784.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..125561	125561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T98373 WP_000775238.1 NZ_CP027367:c98844-98683 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T98373 NZ_CP027367:c98844-98683 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 214 bp

>AT98373 NZ_CP027367:c99103-98890 [Escherichia coli]
ACTGATTGCCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCTGGTATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin