Detailed information of TA system TA098367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4563938..4564540
• Replicon: chromosome
• Accession: NZ_CP027366
• Organism: Escherichia coli strain 89-3156

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: C6P65_RS23780
• Protein ID: WP_000897305.1
• Coordinates: 4563938..4564249 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6P65_RS23785
• Protein ID: WP_000356397.1
• Coordinates: 4564250..4564540 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P65_RS23755	4559852..4560451	+	600	WP_001295269.1	glucose-1-phosphatase	-
C6P65_RS23760	4560445..4561317	+	873	WP_000920762.1	virulence factor BrkB family protein	-
C6P65_RS23765	4561314..4561751	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C6P65_RS23770	4561796..4562737	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C6P65_RS23775	4562801..4563709	-	909	WP_001162704.1	alpha/beta hydrolase	-
C6P65_RS23780	4563938..4564249	+	312	WP_000897305.1	hypothetical protein	Toxin
C6P65_RS23785	4564250..4564540	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C6P65_RS23795	4565126..4565344	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
C6P65_RS23800	4565563..4565805	+	243	WP_001087409.1	hypothetical protein	-
C6P65_RS23805	4566135..4567064	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
C6P65_RS23810	4567061..4567696	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C6P65_RS23815	4567693..4568595	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98367 WP_000897305.1 NZ_CP027366:4563938-4564249 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98367 NZ_CP027366:4563938-4564249 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98367 WP_000356397.1 NZ_CP027366:4564250-4564540 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98367 NZ_CP027366:4564250-4564540 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7