Detailed information of TA system TA098326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1870437..1871164
• Replicon: chromosome
• Accession: NZ_CP027362
• Organism: Escherichia coli strain 95-3192

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: C6P51_RS10210
• Protein ID: WP_000547563.1
• Coordinates: 1870853..1871164 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C6P51_RS10205
• Protein ID: WP_000126294.1
• Coordinates: 1870437..1870856 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P51_RS10185	1865486..1866013	-	528	WP_001078777.1	electron transport protein HydN	-
C6P51_RS10190	1866162..1867172	-	1011	WP_032161993.1	DNA-binding transcriptional regulator AscG	-
C6P51_RS10195	1867433..1868890	+	1458	WP_001107816.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C6P51_RS10200	1868899..1870323	+	1425	WP_000110310.1	6-phospho-beta-glucosidase AscB	-
C6P51_RS10205	1870437..1870856	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
C6P51_RS10210	1870853..1871164	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
C6P51_RS10215	1871326..1872081	-	756	WP_001400787.1	hypothetical protein	-
C6P51_RS10220	1872124..1872591	-	468	WP_000132960.1	hydrogenase maturation peptidase HycI	-
C6P51_RS10225	1872584..1872994	-	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
C6P51_RS10230	1872991..1873758	-	768	WP_000067400.1	formate hydrogenlyase subunit HycG	-
C6P51_RS10235	1873758..1874300	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
C6P51_RS10240	1874310..1876019	-	1710	WP_001288147.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T98326 WP_000547563.1 NZ_CP027362:c1871164-1870853 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T98326 NZ_CP027362:c1871164-1870853 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT98326 WP_000126294.1 NZ_CP027362:c1870856-1870437 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT98326 NZ_CP027362:c1870856-1870437 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACAATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22