Detailed information of TA system TA098309

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 4421378..4421749
• Replicon: chromosome
• Accession: NZ_CP027361
• Organism: Escherichia coli strain 2014C-4639

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: RR62_RS22545
• Protein ID: WP_042853000.1
• Coordinates: 4421378..4421572 (+)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	4421571..4421749 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RR62_RS22515 (4416456)	4416456..4416608	+	153	WP_001427316.1	DUF1391 family protein	-
RR62_RS22520 (4417029)	4417029..4417250	+	222	WP_000560218.1	killing protein KilR	-
RR62_RS22525 (4417250)	4417250..4417420	+	171	WP_001427414.1	YdaE family protein	-
RR62_RS22530 (4417494)	4417494..4417769	+	276	WP_001307773.1	hypothetical protein	-
RR62_RS22535 (4417868)	4417868..4420519	+	2652	WP_000105101.1	exodeoxyribonuclease VIII	-
RR62_RS22540 (4420512)	4420512..4421321	+	810	WP_000166317.1	recombination protein RecT	-
RR62_RS22545 (4421378)	4421378..4421572	+	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
- (4421571)	4421571..4421749	-	179	NuclAT_1	-	Antitoxin
- (4421571)	4421571..4421749	-	179	NuclAT_1	-	Antitoxin
- (4421571)	4421571..4421749	-	179	NuclAT_1	-	Antitoxin
- (4421571)	4421571..4421749	-	179	NuclAT_1	-	Antitoxin
RR62_RS22550 (4421565)	4421565..4421753	+	189	WP_001356607.1	DUF1187 family protein	-
RR62_RS22555 (4421860)	4421860..4422141	+	282	WP_001443927.1	excisionase	-
RR62_RS22560 (4422107)	4422107..4423222	+	1116	WP_001189089.1	phage integrase Arm DNA-binding domain-containing protein	-
RR62_RS22565 (4423575)	4423575..4423916	-	342	WP_000976492.1	YebY family protein	-
RR62_RS22570 (4423929)	4423929..4424798	-	870	WP_032341845.1	copper homeostasis membrane protein CopD	-
RR62_RS22575 (4424802)	4424802..4425176	-	375	WP_000204699.1	CopC domain-containing protein YobA	-
RR62_RS22580 (4425315)	4425315..4425545	+	231	WP_000916763.1	DNA polymerase III subunit theta	-
RR62_RS22585 (4425647)	4425647..4426303	+	657	WP_000011658.1	carbon-nitrogen hydrolase family protein YobB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleC	4371193..4429046	57853

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T98309 WP_042853000.1 NZ_CP027361:4421378-4421572 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T98309 NZ_CP027361:4421378-4421572 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACAGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT98309 NZ_CP027361:c4421749-4421571 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin