Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2467788..2468323 Replicon chromosome
Accession NZ_CP027361
Organism Escherichia coli strain 2014C-4639

Toxin (Protein)


Gene name yafQ Uniprot ID A0A0F3UZU8
Locus tag RR62_RS12125 Protein ID WP_032183258.1
Coordinates 2467788..2468075 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag RR62_RS12130 Protein ID WP_000729704.1
Coordinates 2468063..2468323 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RR62_RS12110 (2465370) 2465370..2465948 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
RR62_RS12115 (2466154) 2466154..2466921 + 768 WP_000333380.1 class II glutamine amidotransferase -
RR62_RS12120 (2466892) 2466892..2467632 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
RR62_RS12125 (2467788) 2467788..2468075 - 288 WP_032183258.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
RR62_RS12130 (2468063) 2468063..2468323 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
RR62_RS12135 (2468509) 2468509..2469282 + 774 WP_000543897.1 C40 family peptidase -
RR62_RS12140 (2469339) 2469339..2469695 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
RR62_RS12145 (2469688) 2469688..2469966 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
RR62_RS12150 (2470071) 2470071..2472164 - 2094 WP_001140806.1 flagellar biosynthesis protein FlhA -
RR62_RS12155 (2472148) 2472148..2473287 - 1140 WP_000785656.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(7-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11166.96 Da        Isoelectric Point: 10.2267

>T98294 WP_032183258.1 NZ_CP027361:c2468075-2467788 [Escherichia coli]
IRNLNQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDK
LLRFERTGTHAALFG

Download         Length: 288 bp

>T98294 NZ_CP027361:c2468075-2467788 [Escherichia coli]
ATTAGGAATTTAAATCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAA
GGATATGAATAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACC
CGCTGCAAGGTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAA
CTTTTACGATTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT98294 WP_000729704.1 NZ_CP027361:c2468323-2468063 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT98294 NZ_CP027361:c2468323-2468063 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F3UZU8

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References