Detailed information of TA system TA098288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1216657..1217259
• Replicon: chromosome
• Accession: NZ_CP027361
• Organism: Escherichia coli strain 2014C-4639

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: RR62_RS06210
• Protein ID: WP_000897305.1
• Coordinates: 1216948..1217259 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RR62_RS06205
• Protein ID: WP_000356397.1
• Coordinates: 1216657..1216947 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RR62_RS06180 (1212203)	1212203..1212838	+	636	WP_000829008.1	formate dehydrogenase cytochrome b556 subunit	-
RR62_RS06185 (1212835)	1212835..1213764	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
RR62_RS06190 (1214164)	1214164..1215144	+	981	WP_000399648.1	IS110-like element IS621 family transposase	-
RR62_RS06195 (1215373)	1215373..1215615	-	243	WP_001086390.1	protein YiiF	-
RR62_RS06200 (1215834)	1215834..1216052	-	219	WP_001295676.1	CopG family transcriptional regulator	-
RR62_RS06205 (1216657)	1216657..1216947	-	291	WP_000356397.1	NadS family protein	Antitoxin
RR62_RS06210 (1216948)	1216948..1217259	-	312	WP_000897305.1	hypothetical protein	Toxin
RR62_RS06215 (1217488)	1217488..1218396	+	909	WP_001162704.1	alpha/beta hydrolase	-
RR62_RS06220 (1218460)	1218460..1219401	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
RR62_RS06225 (1219446)	1219446..1219883	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
RR62_RS06230 (1219880)	1219880..1220752	-	873	WP_000920762.1	virulence factor BrkB family protein	-
RR62_RS06235 (1220746)	1220746..1221345	-	600	WP_001315111.1	glucose-1-phosphatase	-
RR62_RS06240 (1221444)	1221444..1222229	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1214164..1215144	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T98288 WP_000897305.1 NZ_CP027361:c1217259-1216948 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T98288 NZ_CP027361:c1217259-1216948 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT98288 WP_000356397.1 NZ_CP027361:c1216947-1216657 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT98288 NZ_CP027361:c1216947-1216657 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7