Detailed information of TA system TA098284

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 239350..240077
• Replicon: chromosome
• Accession: NZ_CP027361
• Organism: Escherichia coli strain 2014C-4639

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UEW4
• Locus tag: RR62_RS01480
• Protein ID: WP_000547559.1
• Coordinates: 239766..240077 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RR62_RS01475
• Protein ID: WP_000126294.1
• Coordinates: 239350..239769 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RR62_RS01455 (234400)	234400..234927	-	528	WP_001078781.1	electron transport protein HydN	-
RR62_RS01460 (235076)	235076..236086	-	1011	WP_001377591.1	DNA-binding transcriptional regulator AscG	-
RR62_RS01465 (236346)	236346..237803	+	1458	WP_001107856.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
RR62_RS01470 (237812)	237812..239236	+	1425	WP_000110313.1	6-phospho-beta-glucosidase AscB	-
RR62_RS01475 (239350)	239350..239769	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
RR62_RS01480 (239766)	239766..240077	-	312	WP_000547559.1	type II toxin-antitoxin system HigB family toxin	Toxin
RR62_RS01485 (240251)	240251..240994	-	744	WP_001341829.1	hypothetical protein	-
RR62_RS01490 (241037)	241037..241507	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
RR62_RS01495 (241500)	241500..241910	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
RR62_RS01500 (241907)	241907..242674	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
RR62_RS01505 (242674)	242674..243216	-	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
RR62_RS01510 (243226)	243226..244935	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (24-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12450.11 Da
• Isoelectric Point: 9.7249

>T98284 WP_000547559.1 NZ_CP027361:c240077-239766 [Escherichia coli]
MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T98284 NZ_CP027361:c240077-239766 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCATCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT98284 WP_000126294.1 NZ_CP027361:c239769-239350 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT98284 NZ_CP027361:c239769-239350 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0F3V163