Detailed information of TA system TA098265

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2692097..2692791
• Replicon: chromosome
• Accession: NZ_CP027355
• Organism: Escherichia coli strain 2013C-4991

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: C6P55_RS14920
• Protein ID: WP_001263493.1
• Coordinates: 2692097..2692495 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: C6P55_RS14925
• Protein ID: WP_000554757.1
• Coordinates: 2692498..2692791 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C6P55_RS14890	2687097..2688341	-	1245	WP_000189541.1	esterase FrsA	-
-	2687757..2687837	-	81	NuclAT_10	-	-
-	2687757..2687837	-	81	NuclAT_10	-	-
-	2687757..2687837	-	81	NuclAT_10	-	-
-	2687757..2687837	-	81	NuclAT_10	-	-
C6P55_RS14895	2688433..2688891	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
C6P55_RS14900	2689152..2690609	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
C6P55_RS14905	2690666..2691187	-	522	Protein_2717	peptide chain release factor H	-
C6P55_RS14910	2691186..2691389	-	204	Protein_2718	RNA ligase RtcB family protein	-
C6P55_RS14915	2691635..2692087	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
C6P55_RS14920	2692097..2692495	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C6P55_RS14925	2692498..2692791	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C6P55_RS14930	2692843..2693898	-	1056	WP_001226164.1	DNA polymerase IV	-
C6P55_RS14935	2693969..2694754	-	786	WP_162541364.1	putative lateral flagellar export/assembly protein LafU	-
C6P55_RS14940	2694726..2696438	+	1713	Protein_2724	flagellar biosynthesis protein FlhA	-
C6P55_RS14950	2696864..2697361	-	498	WP_033802985.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T98265 WP_001263493.1 NZ_CP027355:c2692495-2692097 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T98265 NZ_CP027355:c2692495-2692097 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT98265 WP_000554757.1 NZ_CP027355:c2692791-2692498 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT98265 NZ_CP027355:c2692791-2692498 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6